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Sherlin, Herald J.
- Light Green Stain, Nuclear Fast Red Stain used an Alternative Routine Haematoxylin and Eosin Stain
Abstract Views :174 |
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Authors
Aiman Zafar
1,
Pratibha Ramani
1,
Herald J. Sherlin
1,
Gheena
1,
R. Abhilasha
1,
Giffrina Jayaraj
1,
K. R. Don
1,
S. Archana
1
Affiliations
1 Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, IN
1 Department of Oral and Maillofacial Pathology, Saveetha Dental College, Saveetha University, 162, poonamalle High Road ,Chennai -600077,Tamil Nadu, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 1 (2019), Pagination: 79-82Abstract
Objective:-1)To compare the intensity of light green stain as cytoplasm and nuclear fast red as nuclear stain compare with routine haematoxylin and eosin stain.2)To evaluate the accuracy of light green stain and nuclear fast red as an alternative stain used in cytology. Materials and methods: 30 buccal smears were collected from 10 healthy patients. they were categorized into 3 groups according to type stains were used. Result: this present study showed statically significant, p value >0.001.Conclusion:Several modifications have been made to improve their efficiency, There has been a rising need for efficient, accurate and less complex staining procedures is requiredKeywords
Stains , Modified Stains.References
- Anderson J. (2011). An introduction to Routine and special staining. Retrieved on August 18, 2014 from. Godwin, A. (2011).
- Histochemical uses of haematoxylin-a review. Retrieved August 18, 2014 From www.arpapress.comHarris, T. J., & McCormick, F. (2010).
- The molecular pathology of cancer. Nat Rev Clin Oncol, 7(5), 251-256.
- Liquid Paraffin as a Rehydrant for Air Dried Buccal Smear
Abstract Views :177 |
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Authors
R. Hannah
1,
Pratibha Ramani
1,
M. P. Brundha
2,
Herald J. Sherlin
1,
Gheena Ranjith
1,
Abilasha Ramasubramanian
1,
Gifrina Jayaraj
1,
K. R. Don
1,
S. Archana
1
Affiliations
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
1 Department of oral and Maxillofacial Pathology and Microbiology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077., IN
2 Department of General Pathology, Saveetha Dental College and Hospitals, SIMATS, No-24, Moorthy Nagar, Chettiaragaram, Thiruverkadu Post, Chennai -600077, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 3 (2019), Pagination: 1197-1200Abstract
Aim: To assess the efficacy of liquid paraffin as a rehydrant for air dried buccal smear based on the nuclear and cytoplasmic details. Background: Buccal smear is useful for diagnosing Malignancy, Fungal infection, Viral infection and Vesicullobullous dermatoses. The routine practice is to wetfixthe smear and send them to the laboratory for staining and evaluation by a cytopathologist. But drying of smears is inevitable, especially when the aspirate is less and when there is improper fixation. This can cause drying artifacts. An alternative method for overcoming this problem is intentional air drying followed by rehydration. Many rehydrants have been experimented upon. The most common rehydrant being saline. The present study is done to check the efficacy of liquid paraffin as a rehydrant. Material and methods: 2 sets of 20 buccal smears were collected from the patients. One set air dried for 24 hours and the other wet fixed. Conventional pap staining was then carried out. The slides were examined by two observers for preservation of Nuclear and cytoplasmic details based on the semi quantitative scoring system. Results: Excellent nuclear details were seen in 45% of air dried smear compared to 25%in routine wet fixation. The cytoplasmic details of air dried smear and routine wet fixed smear was the same. Conclusion: Liquid paraffin as a rehydrant has shown promising results and the nuclear details were found to marginally better than in routine wet fixed smear. Air dried buccal smears can be used in routine practice especially in rural areas where there is limited access to laboratories and for mass screening.Keywords
Buccal smear, Rehydration, Liquid Paraffin.References
- Hoda R.S., Hoda S.A. Artifacts, contaminants and incidental findings. In: Fundamentals of Pap Test Cytology. Humana Press 2007; 179-186.
- Lencioni LJ, Staffieri JJ, Cardinnet LJ. Vaginal and urinary sediment smear staining technique without previous fixation; adapted to Papanicolaou’s and Shorr’s staining methods. J Lab Clin Med.1954; 44(4): 595-9.
- Chan JKC, Kung ITM. Rehydration of airdried smears with normal saline: application in fine-needle aspiration cytologic examination. Am J Clin Pathol 1988; 89(1):30-4.
- M. S. Israel, L.F. Young. Use of liquid paraffin in the preservation of pathological specimens. J Clin Path.1978; 31(5):499-500.
- Crothers, Barbara and Henry, Michael and Firat, Pinar and Hamper, Ulrike. Nondiagnostic/Unsatisfactory: The Bethesda System for Reporting Thyroid Cytopathology. 2010.
- Ahmed HG, Tom MA. The consequence of delayed fixation on subsequent preservation of urine cells. Oman Medical Journal. 2011; 26(1):14-8.
- Zare –Mirzaie, K. Kalili-Alam and M. Abolhasani. Rehydration of air-dried cervical smears: An alternative to routine wet fixation. Acta Medica Iranica 2007; 45(5):365-68.
- Dietary Habits Leading to Recurrent Aphthous Ulcers-A Survey
Abstract Views :274 |
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Authors
Affiliations
1 Department of Oral Pathology, Saveetha Dental College, SIMATS, Saveetha University, Chennai, IN
1 Department of Oral Pathology, Saveetha Dental College, SIMATS, Saveetha University, Chennai, IN
Source
Research Journal of Pharmacy and Technology, Vol 12, No 7 (2019), Pagination: 3479-3482Abstract
Background: Aphthous ulcers are ulcers that form on the oral mucous membranes. They are known as aphthae, aphthous stomatitis and also canker sores. Aphthous ulcers are typically recurrent round or oval sores or ulcers inside the mouth found on the inside of the lips and cheeks or underneath the tongue. Patients often complain about very painful wounds inside their mouth that prevent them from eating food. The present study was designed to identify the dietary factors that trigger occurrence of RAS. Materials and Method: A qualitative survey was conducted through a structured Questionnaire presented to individuals with RAS visiting a Dental college and Hospital in Chennai between July to decemebr 2017. The results were expressed in percentage. Result: The study results revealed that 40% of them had a family history of RAS. 85% were anemic and stress was a precipitating factor in all the individuals (100%). Buccal mucosa (80%) was the most common site involved. 21% had RAS during Menstruation. RAS was triggered by consumption of gluten rich food in 80% of the individuals and spicy food in all the participants (100%). Conclusion: Stress, fatigue, everyday consumption of gluten rich diet, and spicy food trigger the development of RAS. This may be prevented by modifying their dietary habits.Keywords
Aphthous, Diet, Gluten, Anemia, Soft Drinks.References
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- O’Mahony C, O’ Farrelly C, Weir DG. Gluten-sensitive oral ulceration in the absence of coeliac disease. Gut 1985, 26: A1137. 11. Bassel Tarakji, Kusai Baroudi, and Yaser Kharma. The effect of dietary habits on the development of the recurrent aphthous stomatitis:. Niger Med J. 2012 Jan-Mar; 53(1): 9–11.
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- Porter SR, Scully C, Flint S. Hematologic status in recurrent aphthous stomatitis compared with other oral disease. Oral Surg Oral Med Oral Pathol 1988, 66(1):41-4.
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